Since dideoxy sequencing is based on the chain termination,why are normal deoxynucleotides also included in the reaction?
A)to enhance the chain termination ability of the deoxynucleotides
B)to provide a substrate for DNA polymerase
C)to produce a range of DNA synthesis products that terminate at every occurrence of a particular base
D)to create DNA synthesis products long enough to allow running a gel

Question

Quizplus · Accepted Answer

Normal deoxynucleotides are included to allow DNA synthesis to continue until a dideoxynucleotide is incorporated, resulting in termination. This creates a range of DNA fragments of varying lengths, each terminating at a different occurrence of the base, which is necessary for sequencing.

Since Dideoxy Sequencing Is Based on the Chain Termination,why Are