The following table from the Venter et al.paper presents the numbers of different types of genes discovered in this study.

-Refer to the paragraph on the Venter et al.paper and Table 20.1 Because DNAs for sequencing were chosen at random with no way of knowing which organism they came from,how could genes be identified?
A)by searching for ORFs,especially those with translation start and stop sites
B)by searching for similarity between predicted ORFs and those in existing databases
C)by searching for exons bounded by consensus donor and acceptor splice sites (those that mark exon/intron boundaries)
D)by searching for ORFs,particularly those with translation start and stop sites,as well as for similarity between predicted ORFs and those in existing databases
E)by searching for ORFs,particularly those with translation start and stop sites;for similarity between predicted ORFs and those in existing databases;and for exons bounded by consensus donor and acceptor splice sites (those that mark exon/intron boundaries)

Question

The following table from the Venter et al.paper presents the numbers of different types of genes discovered in this study.
 
-Refer to the paragraph on the Venter et al.paper and Table 20.1 Because DNAs for sequencing were chosen at random with no way of knowing which organism they came from,how could genes be identified?
A)by searching for ORFs,especially those with translation start and stop sites 
B)by searching for similarity between predicted ORFs and those in existing databases 
C)by searching for exons bounded by consensus donor and acceptor splice sites (those that mark exon/intron boundaries) 
D)by searching for ORFs,particularly those with translation start and stop sites,as well as for similarity between predicted ORFs and those in existing databases 
E)by searching for ORFs,particularly those with translation start and stop sites;for similarity between predicted ORFs and those in existing databases;and for exons bounded by consensus donor and acceptor splice sites (those that mark exon/intron boundaries)

Quizplus · Accepted Answer

The paragraph states that genes were identified by searching for ORFs (open reading frames) with translation start and stop sites, as well as for similarity between predicted ORFs and those in existing databases. Therefore, choice D is the best option as it includes both of these methods. There is no mention of searching for exons with consensus splice sites, so choices A, B, and E can be eliminated.

The Following Table from the Venter Et Al