Primary inoculation to a Loeffler slant, overnight incubation, and subculture of any growth to cystine-tellurite blood agar is no longer recommended for recovery of

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Selective and differential media for C. diphtheriae should be used if diphtheria is suspected. The two media commonly used for this purpose are cystine-tellurite blood agar and modified Tinsdale's agar. In addition, Loeffler medium, containing serum and egg, stimulates the growth of C. diphtheriae and the production of metachromatic granules within the cells. Primary inoculation of throat swabs to Loeffler serum slants is no longer recommended because of the inevitable overgrowth of other bacteria.

Primary Inoculation to a Loeffler Slant, Overnight Incubation, and Subculture