The primary plating media for inoculating a wound aspirate for anaerobic culture may include

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The primary plating media for inoculating anaerobic specimens includes a nonselective anaerobic blood agar and one or all of the following selective media: Bacteroides bile esculin agar (BBE), laked kanamycin-vancomycin blood agar (LKV), or anaerobic phenylethyl alcohol agar (PEA). In addition, aerobic 5% sheep blood agar, chocolate agar, and MacConkey agar are set up because most anaerobic infections are polymicrobic and may include aerobic or facultative anaerobic bacteria. A backup broth, usually thioglycollate, to enrich for small numbers of anaerobes may also be included on tissues and other sterile specimens.

The Primary Plating Media for Inoculating a Wound Aspirate for Anaerobic