Incubation conditions for anaerobic cultures include

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In general, anaerobic cultures should not be exposed to oxygen until after 48 hours, because anaerobes are most sensitive to oxygen during their log phase of growth. Also, colony morphology often changes dramatically between 24 and 48 hours. Plates that show no growth at 48 hours should be incubated for at least 5 days before discarding. The thioglycollate broth can be incubated anaerobically with the cap loose or aerobically with the cap tight.

Incubation Conditions for Anaerobic Cultures Include