To inoculate test media, such as biochemical tests and pigmentation and growth rate determinations, the laboratorian would

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Regardless of whether molecular or phenotypic methods are employed, when growth is detected, broth subcultures of colonies growing on solid media (several colonies inoculated to Middlebrook 7H9 broth [5 mL] and incubated at 35°C for 5 to 7 days with daily agitation to enhance growth) or liquid media is then used for pigmentation and growth rate determinations as well as to inoculate all test media for biochemical tests, if used. In some instances, additional cultures may be inoculated and then incubated at different temperatures when more definitive identification is needed.

To Inoculate Test Media, Such as Biochemical Tests and Pigmentation