The 3-day test that is useful for identifying the rapid-growers M.fortuitum and M.chelonae and that measures the rate by which this enzyme breaks down phenolphthalein disulfate into phenolphthalein and other salts is

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The enzyme arylsulfatase is present in most mycobacteria. Test conditions can be varied to differentiate different forms of the enzyme. The rate by which this enzyme breaks down phenolphthalein disulfate into phenolphthalein (which forms a red color in the presence of sodium bicarbonate) and other salts helps differentiate certain strains of mycobacteria. The 3-day test is particularly useful for identifying the potentially pathogenic rapid-growers M. fortuitum and M. chelonae.

The 3-Day Test That Is Useful for Identifying the Rapid-Growers