How does the DNA enzyme topoisomerase contribute to DNA replication?
A)Unwinds the double helix and separates the double-stranded DNA
B)Creates a "nick" in the DNA supercoils, allowing them to straighten before replication
C)Initiates DNA synthesis in multiple sites down the strand, making the process more efficient
D)Connects and links the individual pieces of newly synthesized DNA to form a single strand

Question

Quizplus · Accepted Answer

Replication of DNA is performed when double-stranded DNA is separated and each strand is used as a template to guide the correct construction of a complementary strand. Synthesis of new DNA begins at multiple sites, creating many short pieces. The enzyme DNA ligase connects or links the individual pieces of newly synthesized DNA during replication, forming a new single strand complementary to the template strand. The enzyme DNA helicase unwinds the double helix and initially separates the ds-DNA. The group of topoisomerase enzymes creates a "nick" in the supercoils of ds-DNA, allowing them to loosen so that eventually the two strands can separate. The enzyme primase is responsible for initiating DNA synthesis in multiple sites down the single strand being copied.

How Does the DNA Enzyme Topoisomerase Contribute to DNA Replication