You wish to obtain a pure mitochondria sample from lysed cells.The best way to obtain this sample would be
A)centrifugation.
B)chromatography.
C)polyacrylamide gel electrophoresis.
D)agarose gel electrophoresis.
E)both choices A and C

Question

Quizplus · Accepted Answer

Centrifugation is the most efficient method for isolating pure mitochondria from lysed cells. Mitochondria, being denser and larger than other cellular components, can easily be isolated by differential centrifugation. This method involves subjecting the cell lysate to a series of centrifugation steps at progressively increasing speeds, which allows the separation of different cellular components based on their size and density. The mitochondrial fraction can then be further purified by using a sucrose density gradient or other techniques. Chromatography and gel electrophoresis are not suitable for isolation of large organelles like mitochondria.

You Wish to Obtain a Pure Mitochondria Sample from Lysed