Inactivation of a fluorescent dye in a very concentrated spot on a cell so that the fluidity of membranes can be visualized is called
A)liposome formation.
B)SDS-PAGE.
C)photobleaching.
D)the freeze-fracture technique.
E)ferritin-conjugated lectins.

Question

Quizplus · Accepted Answer

Inactivation of a fluorescent dye in a very concentrated spot on a cell so that the fluidity of membranes can be visualized is called photobleaching. This method involves using intense illumination to cause irreversible inactivation of the fluorophore, which creates a dark spot on the otherwise fluorescent sample. The surrounding, unaffected fluorophores will then diffuse into the bleached spot, indicating the mobility of the membrane. This method is often used in fluorescence recovery after photobleaching (FRAP) experiments to track macromolecules in living cells.

Inactivation of a Fluorescent Dye in a Very Concentrated Spot