A means of detecting the presence of specific carbohydrate moieties on glycoproteins is
A)SDS-PAGE.
B)the freeze-fracture technique.
C)photobleaching.
D)ferritin-conjugated lectins.
E)liposome formation.

Question

Quizplus · Accepted Answer

Ferritin-conjugated lectins are used to detect the presence of specific carbohydrate moieties on glycoproteins. Lectins are proteins or glycoproteins that bind specifically to carbohydrates, and when they are conjugated to the iron-storage protein ferritin, they can be used as probes to detect specific sugars on glycoproteins. The lectin-ferritin complexes can be visualized by electron microscopy, and the location of the complexes can provide information about the distribution of specific carbohydrates on glycoproteins. SDS-PAGE is a method of separating proteins based on their size, and the freeze-fracture technique is used to examine the structure of cell membranes. Photobleaching is a technique used to study the movement of molecules in cells, and liposome formation is a method of preparing artificial membranes.

A Means of Detecting the Presence of Specific Carbohydrate Moieties