The normal gene for hemoglobin was isolated,cloned,and subjected to restriction enzyme digestion with BamHI.Following gel electrophoresis,three bands are routinely obtained.However,following the same procedure with the hemoglobin gene from an individual with sickle-cell anemia,four bands are routinely obtained.These data suggest that
A)the mutation associated with sickle-cell anemia results in DNA that breaks easily.
B)the mutation associated with sickle-cell anemia results in the loss of a BamHI cut site.
C)the mutation associated with sickle-cell anemia results in several new enzyme cut sites for a variety of restriction enzymes.
D)the mutation associated with sickle-cell anemia results in the gain of a BamHI cut site.
E)the assay was not performed correctly.

Question

Quizplus · Accepted Answer

The fact that the sickle-cell anemia sample has four bands instead of three suggests that the mutation has resulted in the addition of another BamHI restriction enzyme cut site. This could be due to a deletion or insertion of nucleotides in the gene sequence, leading to a change in the location of the cut site.

The Normal Gene for Hemoglobin Was Isolated,cloned,and Subjected to Restriction