In the Sanger method of DNA sequencing,incorporation of the nucleotides stops because
A)the dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis.
B)the dideoxynucleotide analogs have no 5'-OH group to continue DNA synthesis.
C)the dideoxynucleotide analogs have no 2'-OH group to continue DNA synthesis.
D)the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
E)the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.

Question

Quizplus · Accepted Answer

The dideoxynucleotide analogs used in the Sanger method of DNA sequencing lack a 3'-OH group, which is required for DNA synthesis to continue. This results in the termination of the elongation of the DNA chain when a dideoxynucleotide is incorporated, thereby allowing the determination of the sequence.

In the Sanger Method of DNA Sequencing,incorporation of the Nucleotides