A Variety of E

A variety of E.coli clones have been isolated,and the DNA has been extracted,digested with restriction enzymes,denatured,separated by gel electrophoresis,and transferred to nylon membrane by the Southern blot procedure.A radioactive probe is used that hybridizes with a gene of interest.The DNA corresponding to a single clone yields a single DNA band after probing the blot.Based on this information only,

A) all of the clones tested, except the one described, have the gene of interest.
B) only the one clone described has the gene of interest.
C) the gene of interest is located entirely on the DNA band visualized.
D) the part of the gene recognized by the probe is located on the DNA band visualized.
E) both choices B and D