You are using genetic engineering to design a protein that needs to be synthesized in the cytoplasm and then localized in the nucleus of the cell.To accomplish this,one could add
A)a nuclear localization signal into the DNA that will be present in the mature protein.
B)the compound during mitosis, at a time when the nuclear membrane is dispersed.
C)a nucleotide or few nucleotides to the protein.
D)a series of specific polysaccharides to the carboxyterminal end of the protein.
E)all of the above

Question

Quizplus · Accepted Answer

The addition of a nuclear localization signal (NLS) is the best option for targeting the protein to the nucleus. An NLS is a short amino acid sequence in the protein that specifically interacts with nuclear import receptors, allowing the protein to be transported into the nucleus. Adding nucleotides or polysaccharides would not be effective for targeting the protein to the nucleus, and adding the compound during mitosis would only be effective during a specific phase of the cell cycle.

You Are Using Genetic Engineering to Design a Protein That