In the process of isolating DNA for transcription and translation,you digest the DNA with BamHI and ligate the gene into a plasmid vector.However,even though all of the controls work,no transcription is obtained using your fragment.Working backward,you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene.It is possible that during restriction digestion
A)the termination signal was removed.
B)the RNA polymerase became inactivated.
C)primase could not bind.
D)the promoter was removed.
E)none of the above

Question

Quizplus · Accepted Answer

The promoter is typically located at the beginning of a gene, therefore if approximately 250 base pairs were removed from the 5' end of the gene during restriction digestion, it is possible that the promoter was removed. Without a promoter, RNA polymerase cannot bind and initiate transcription.

In the Process of Isolating DNA for Transcription and Translation,you