In working with a potentially new antibiotic,you are attempting to determine where in prokaryotic translation this antibiotic has its effect.You have determined thus far that it interacts with a translational factor but need to identify which one.If this antibiotic interfered with elongation,which of the following factors could be a potential site of action?
A)IF3 interaction with an aminoacyl tRNA
B)EF-Tu placement of N-formylmethionine onto the ribosome
C)EF-Tu interaction with an aminoacyl tRNA
D)EF-Ts translocation of tRNAs from the A site to the P site
E)all of the above

Question

Quizplus · Accepted Answer

EF-Tu is responsible for placing aminoacyl tRNAs onto the ribosome during elongation, making it the most likely site of action for an antibiotic that interferes with this process. IF3 is involved in the initiation of translation, while EF-Ts is involved in the regeneration of EF-Tu, and neither of these factors play a direct role in elongation.

In Working with a Potentially New Antibiotic,you Are Attempting to Determine