In a mutant E.coli strain,there was a mutation in the malate dehydrogenase gene that resulted in a stop codon at amino acid position 14.From subcultures of this strain,a colony was obtained that was capable of making malate dehydrogenase.Your approach to examining this phenomenon should be to examine
A)the DNA sequence to see if a reversion has occurred.
B)the DNA sequence to see if a suppresor mutation has occurred.
C)other proteins to see if other stop codons are ignored, indicating the presence of a suppresor tRNA.
D)the DNA and plasmids for the incorporation of a second complete copy of the malate dehydrogenase gene.
E)all of the above

Question

Quizplus · Accepted Answer

A comprehensive approach is needed to determine the mechanism by which the colony regained the ability to produce malate dehydrogenase. This includes checking for reversion mutations, suppressor mutations, suppressor tRNA activity, and the presence of additional gene copies.

In a Mutant E