The enzymes transketolase and transaldolase of the pentose phosphate pathway are each very specific for only one substrate.

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Both transketolase and transaldolase of the pentose phosphate pathway are bifunctional enzymes that use multiple substrates. Transketolase catalyzes the transfer of a two-carbon unit from a ketose donor to an aldose acceptor, producing a different ketose and aldose. Transaldolase catalyzes the transfer of a three-carbon unit from a ketose donor to an aldose acceptor, producing a different ketose and aldose.

The Enzymes Transketolase and Transaldolase of the Pentose Phosphate Pathway