The recycling of NAD allows glycolysis to continue in the absence of oxygen.

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NAD+ is a coenzyme involved in redox reactions in cells. In the absence of oxygen, glycolysis cannot proceed because the electron transport chain, which uses oxygen as a final electron acceptor, cannot operate. However, the recycling of NAD+ to NADH during glycolysis allows for the continued production of ATP through substrate-level phosphorylation. This enables glycolysis to continue without oxygen, as long as NAD+ is continually regenerated through alternative means such as fermentation.

The Recycling of NAD Allows Glycolysis to Continue in the Absence