In the amino acid analysis the PTC-amino acid derivatives of a hydrolyzed protein are subjected to HPLC.How do you determine which amino acids are present?

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Amino acids have different chemical properties that affect their elution times on an HPLC column. By comparing the elution times of the PTC-amino acid derivatives from the hydrolyzed protein to those of standards for each amino acid, the presence of specific amino acids can be determined.

In the Amino Acid Analysis the PTC-Amino Acid Derivatives of a Hydrolyzed