In gel electrophoresis the molecules with the highest molecular weight migrate the farthest from the starting wells.

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In gel electrophoresis, the molecules with the lowest molecular weight migrate the farthest from the starting wells, and those with the highest molecular weight migrate the least. This is because the gel matrix creates a molecular sieve that impedes the movement of large molecules, allowing smaller ones to move more easily through the pores.

In Gel Electrophoresis the Molecules with the Highest Molecular Weight