The roles of amino acid residues at the active site of enzymes can be determined by removing certain residues using the technique of

Question

Quizplus · Accepted Answer

The technique of site-directed mutagenesis can be used to remove or replace specific amino acid residues at the active site of enzymes. This allows the roles of these residues to be determined by observing the effect on enzyme activity. Specific hydrolysis, covalent binding, and acylation of specific residues do not necessarily involve the removal of specific amino acid residues and are not as targeted as site-directed mutagenesis. Therefore, the best choice to determine the roles of amino acid residues at the active site of enzymes is site-directed mutagenesis.

The Roles of Amino Acid Residues at the Active Site