What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.

Question

Quizplus · Accepted Answer

The sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium is as follows: 
1. Extract plasmid DNA from bacterial cells (III) 
2. Cut the plasmid DNA using restriction enzymes (II) 
3. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments (IV) 
4. Use ligase to seal plasmid DNA to nonplasmid DNA (V) 
5. Transform bacteria with recombinant DNA molecule (I) 
Therefore, the correct sequence is III, II, IV, V, I, and the best choice is C.

What Is the Most Logical Sequence of Steps for Splicing