When centrifuged, blood separates into the following sequence of layers, from lightest to heaviest

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When blood is centrifuged, it separates into layers based on the density of its components. The lightest layer is plasma, which rises to the top. The middle layer is the buffy coat, which contains white blood cells and platelets. The heaviest layer, at the bottom, is the hematocrit, which is made up of red blood cells.

When Centrifuged, Blood Separates into the Following Sequence of Layers