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Nucleotides can be radiolabelled before they are incorporated into newly forming DNA and can therefore be assayed to track their incorporation. In a set of experiments, a student-faculty research team used labelled T nucleotides and introduced these into the culture of dividing human cells at specific times.
-Once they had determined which cells were dividing, the team wanted to use a ?non-radioactive method to track whether various physiological factors (such as food or body temperature)affected the action of the pathogen. Which of the following would be effective, simple, and safe?
A)measuring picograms of DNA
B)measuring numbers of chromosomes
C)measuring numbers of chromatids
D)counting the frequency of cells in mitosis
E)counting newly formed plasma membranes

Question

Quizplus · Accepted Answer

Counting the frequency of cells in mitosis would be the most effective, simple, and safe method to track the incorporation of radiolabelled nucleotides as well as any physiological factors affecting the action of the pathogen. The presence of dividing cells indicates ongoing DNA replication and cell division, making it a reliable marker of cellular activity. Additionally, this method does not involve the use of radioactive materials or external markers, making it safe to use. Measuring picograms of DNA, numbers of chromosomes, numbers of chromatids, and counting newly formed plasma membranes would not provide a reliable or practical means of tracking cellular activity or physiological factors.

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