What must be included, in addition to the coding exons of the gene, in order for the expression of a eukaryotic gene engineered into a bacterial colony?
A)the introns
B)eukaryotic polymerases
C)a bacterial promoter sequence
D)eukaryotic ribosomal subunits
E)eukaryotic tRNAs

Question

Quizplus · Accepted Answer

In order for a eukaryotic gene to be expressed in a bacterial colony, a bacterial promoter sequence must be included. Bacteria and eukaryotes have different types of RNA polymerases, ribosomal subunits, and tRNAs, so including these components would not necessarily facilitate expression in a bacterial colony. The introns, while important for eukaryotic gene expression, are spliced out before the mRNA is exported from the nucleus, so they would not be present in the mRNA that would be used in the bacterial colony.

What Must Be Included, in Addition to the Coding Exons