To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would find more probable success with which of the following methods?
A) the shotgun approach
B) electroporation followed by recombination
C) introducing a plasmid into the cell
D) infecting the mouse cell with a Ti plasmid
E) transcription and translation

Question

Quizplus · Accepted Answer

Electroporation followed by recombination is a commonly used method for introducing foreign DNA into animal cells, including mouse cells. Electroporation uses an electrical pulse to temporarily create pores in the cell membrane, allowing for the uptake of DNA. Recombination then enables the foreign DNA to integrate into the genome of the host cell. The other options listed may also be used, but may have lower success rates or be less specific to the target cells.

To Introduce a Particular Piece of DNA into an Animal