A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A)recombination resulting in replacement of the wild type with the mutated gene
B)use of a microarray to verify continued expression of the original gene
C)replication of the cloned gene using a bacterial plasmid
D)transcription of the cloned gene using cDNA
E)attachment of the mutated gene to an existing mRNA to be translated

Question

Quizplus · Accepted Answer

In vitro mutagenesis involves intentionally introducing mutations into a cloned gene outside of a living organism. To disable the function of the gene, the mutated sequence must replace the wild-type gene via recombination in the cell. This can be achieved through various techniques such as homologous recombination or CRISPR/Cas9 gene editing. The other answer choices do not address the need for the mutated sequence to replace the wild-type gene for gene function to be disabled.

A Researcher Has Used in Vitro Mutagenesis to Mutate a Cloned