For a particular microarray assay (DNA chip), cDNA has been made from the mRNAs of a dozen patients' breast tumour biopsies. The researchers will be looking for
A)a particular gene that is amplified in all or most of the patient samples.
B)a pattern of fluorescence that indicates which cells are overproliferating.
C)a pattern shared among some or all of the samples that indicates gene expression differing from control samples.
D)a group of cDNAs that act differently from those on the rest of the grid.
E)a group of cDNAs that match those in non-breast cancer control samples from the same population.

Question

Quizplus · Accepted Answer

The researchers are looking for a pattern shared among some or all of the samples that indicates gene expression differing from control samples. This is usually done by comparing the fluorescence intensity of each spot on the microarray with that of a control sample. If the intensity is different, it indicates that the gene is expressed differently in the patient samples. This helps in identifying the genes that are involved in breast tumour development and progression.

For a Particular Microarray Assay (DNA Chip), CDNA Has Been