Use the following information to answer the next few questions.
A group of six students has taken samples of their own cheek cells, purified the DNA, and used a restriction enzyme known to cut at zero, one, or two sites in a particular gene of interest.
-Their next two steps, in order, should be
A)use of a fluorescent probe for the gene sequence, then electrophoresis.
B)electrophoresis of the fragments followed by autoradiography.
C)electrophoresis of the fragments, followed by the use of a probe.
D)use of a ligase that will anneal the pieces, followed by PCR.
E)use of reverse transcriptase to make cDNA, followed by electrophoresis.

Question

Quizplus · Accepted Answer

The next step after using a restriction enzyme to cut the gene of interest would be to separate the fragments through electrophoresis. The fragments would then need to be identified, which can be done using a probe that binds to the specific gene of interest. Therefore, the correct order of steps would be electrophoresis followed by the use of a probe.

Use the Following Information to Answer the Next Few Questions