The Following Questions Refer to This Hypothetical Situation

The following questions refer to this hypothetical situation.
A female fly, full of fertilized eggs, is swept by high winds to an island far out to sea. She is the first fly to arrive on this island, and the only fly to arrive in this way. Thousands of years later, her numerous offspring occupy the island, but none of them resembles her. There are, instead, several species, each of which eats only a certain type of food. None of the species can fly, for their flight wings are absent, and their balancing organs (in other words, halteres) are now used in courtship displays. The male members of each species bear modified halteres that are unique in appearance to their species. Females bear vestigial halteres. The ranges of all of the daughter species overlap.
-In each fly species, the entire body segment that gave rise to the original flight wings is missing. The mutation(s) that led to the flightless condition could have

A) duplicated all of the Hox genes in these flies' genomes.
B) altered the nucleotide sequence within a Hox gene.
C) altered the expression of a Hox gene.
D) all three of the above responses
E) Two of the above answers are correct.