Leukocyte surface markers identify cell lineage and are helpful in differentiating neoplastic hematologic disorders.What is one method used to identify these surface markers?
A)Chamber counts
B)Leukocyte differential
C)Cytogenetic methods
D)Flow cytometry

Question

Quizplus · Accepted Answer

Flow cytometry is the preferred method for identifying leukocyte surface markers. Flow cytometry uses fluorescently-labeled antibodies specific to particular cell surface markers to identify and quantify cells expressing those markers. This technique provides highly specific and quantitative information about the phenotype of the cells, allowing for accurate diagnosis and classification of hematologic neoplasms. Chamber counts and leukocyte differentials are non-specific methods for counting and categorizing leukocytes, and cytogenetic methods are used to analyze the genetic abnormalities in leukemic cells but do not provide information on surface markers.

Leukocyte Surface Markers Identify Cell Lineage and Are Helpful in Differentiating