The screening sucrose hemolysis (sugar-water) test is useful in identifying PNH cells by what mechanism?
A) Immunophenotyping
B) Hemoglobin electrophoresis
C) Erythrocyte survival test
D) Complement lysis

Question

Quizplus · Accepted Answer

The screening sucrose hemolysis (sugar-water) test identifies PNH cells by the mechanism of complement lysis. PNH cells lack the complement regulatory proteins CD55 and CD59, making them susceptible to complement-mediated lysis in the presence of sugar-water (hypotonic solution). Therefore, PNH cells will undergo hemolysis faster in sugar-water than normal cells.

The Screening Sucrose Hemolysis (Sugar-Water) Test Is Useful in Identifying