The harvest procedure for chromosomal banding is accomplished by first activating the cells into mitosis. The active cells are stopped in metaphase by the addition and incubation of agents capable of disrupting the spindle apparatus. The swelling of the nucleated cells followed by fixation results in a preparation of final cell suspension on a slide, which is evaluated for quality. If the cells' quality is not optimum, what must be done?

Question

Quizplus · Accepted Answer

If the cells' quality is not optimum, the best solution is to concentrate or dilute the preparation, which can improve the quality of the cells. Starting over with inactivation of the cells may not improve the quality, and aging the preparation or staining the preparation will not address the underlying issue of poor quality cells.

The Harvest Procedure for Chromosomal Banding Is Accomplished by First