Lady Macbeth was burdened with intense guilt after her part in the murder of Duncan.As a result,she washed her hands over and over again,trying to remove the blood she thought she saw on them.Psychologists would call Lady Macbeth's hand-washing:

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Lady Macbeth's hand-washing is a compulsion, which is a repetitive behavior performed in response to an obsessive thought or fear. Lady Macbeth feels compelled to wash her hands repeatedly because she cannot rid herself of the guilt she feels over her role in Duncan's murder.

Lady Macbeth Was Burdened with Intense Guilt After Her Part