What two tests have been mainstays in identification schemes for the non-b-hemolytic, catalase-negative, gram-positive cocci?

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Two tests that have been mainstays in identification schemes for the non-b-hemolytic, catalase-negative, gram-positive cocci are the bile esculin hydrolysis test and the salt tolerance test.The bile esculin test is a two-step test; bacteria must grow in the presence of 40% bile and be able to hydrolyze esculin to produce a positive reaction.Growth in 6.5% sodium chloride broth is used to identify Enterococcus and Aerococcus.

What Two Tests Have Been Mainstays in Identification Schemes for the Non-B-Hemolytic