To Inoculate Triple Sugar Iron (TSI)agar or Kligler's Iron Agar

To inoculate triple sugar iron (TSI) agar or Kligler's iron agar (KIA) ,the laboratory scientist should pick a well-isolated colony with an inoculating needle and:

A) Touch the bottom of the needle to the bottom of the slant portion of the agar and move the needle back and forth across the surface of the slant all the way to the top of the tube.
B) Touch the bottom of the needle to the top of the slant portion of the agar and move the needle back and forth across the surface of the slant all the way to the bottom of the tube.
C) Touch the bottom of the needle to the bottom of the slant portion of the agar and move the needle up the slant in a straight line all the way to the top of the tube.
D) Stab the butt almost all the way to the bottom of the tube, then move the needle back and forth over the surface of the slant all the way to the top of the tube.