One laboratory group repeated the same experiment described by others with the identical bacterial isolate and the same growth medium and conditions used but were unable to achieve the same O.D.at 600 nm.What is the LEAST likely cause for this discrepancy of turbidity measured?
A) One lab used 16 mm wide test tubes and the other used 18 mm wide test tubes.
B) One lab subtracted the yellow color of the growth medium away from the final turbidity reported whereas the other lab used colorless water.
C) One lab vigorously dispersed the biofilm-forming bacteria with vortexing and the other did not.
D) The two labs varied with 1000 m elevation and did not consider the influence of pressure.

Question

Quizplus · Accepted Answer

The difference in elevation and pressure would have a negligible effect on the turbidity measurement of the growth medium, and would therefore be the least likely cause for the discrepancy. Choices A, B, and C all involve differences in experimental procedure that could affect the measured turbidity.

One Laboratory Group Repeated the Same Experiment Described by Others