Diversity is often ________ when ARISA or T-RFLP is used.
A) underestimated
B) overestimated
C) not represented
D) biased

Question

Quizplus · Accepted Answer

ARISA (Automated Ribosomal Intergenic Spacer Analysis) and T-RFLP (Terminal Restriction Fragment Length Polymorphism) rely on PCR amplification of a specific region of DNA and subsequent analysis of the resulting fragment lengths. Due to PCR bias and limitations in fragment separation, these methods can underestimate the true diversity present in a sample.

Diversity Is Often ________ When ARISA or T-RFLP Is Used