DGGE and T-RFLP are both molecular microbial ecology methods that use the polymerase chain reaction to amplify specific genes to assess diversity.

Question

Quizplus · Accepted Answer

Both DGGE and T-RFLP are PCR-based methods used in molecular microbial ecology to amplify specific genes, such as 16S rRNA genes, to assess microbial diversity in environmental samples. DGGE (denaturing gradient gel electrophoresis) separates amplified DNA fragments based on their melting behavior in a denaturing gradient gel, while T-RFLP (terminal restriction fragment length polymorphism) involves digesting the amplified DNA fragments with a restriction enzyme and analyzing the resulting fragment size distribution.

DGGE and T-RFLP Are Both Molecular Microbial Ecology Methods That