The microscopic examination of a Wright-stained blood smear revealed bright red erythrocytes and pale leukocyte nuclei. What is the best explanation for this appearance?

Question

Quizplus · Accepted Answer

When the buffer used in the Wright staining process is too acidic, it leads to the erythrocytes staining too brightly and the leukocyte nuclei appearing too pale. This is because the acidic environment affects the binding of the dye to the cellular components differently, enhancing the red color of erythrocytes and diminishing the staining of leukocyte nuclei.

The Microscopic Examination of a Wright-Stained Blood Smear Revealed Bright