CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.The EWGCCA recommendations for this enumeration include proper technique and interpretation of the flow cytometry analysis.Which of the following standards were recommended for proper acquisition of events?

Question

Quizplus · Accepted Answer

The EWGCCA recommendations state that bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34 should be used for proper acquisition of events. Including platelets and unlysed red blood cells and debris is discouraged, and acquiring only 10 CD34 positive cells is not sufficient for accurate enumeration. Only including CD34 dim staining populations is also not recommended, as it may exclude some CD34-positive cells that are important for enumeration.

CD34 Enumeration Is Useful in Bone Marrow and Peripheral Blood