You suspect a region of DNA contains a promoter and wish to confirm that a sequence of interest binds proteins. Which experiment would you use?
A)in situ hybridization
B)band shift assay
C)pulse-chase assay
D)Southern blotting
E)northern blotting

Question

Quizplus · Accepted Answer

The band shift assay, also known as electrophoretic mobility shift assay (EMSA), is a technique used to confirm protein binding to a specific DNA sequence. This assay involves incubating the DNA sequence with a protein extract and running it on a gel electrophoresis. If the protein binds to the DNA sequence, the mobility of the DNA on the gel will be reduced and a shifted band will be observed. Therefore, in this case, the band shift assay would be the best experiment to confirm protein binding to the region of interest.

You Suspect a Region of DNA Contains a Promoter and Wish