Which assay allows you to identify the exact location of the protein-binding sequence within a promoter?
A)western blotting
B)in situ hybridization
C)pulse-chase assay
D)Southern blotting
E)DNA footprint protection assay

Question

Quizplus · Accepted Answer

The DNA footprint protection assay allows identification of the exact location of a protein-binding sequence within a promoter. This assay involves labeling the ends of the DNA fragment upstream of the promoter and performing a protease digestion followed by gel electrophoresis. The protected fragments can then be sequenced to determine the exact location of the protein-binding sequence.

Which Assay Allows You to Identify the Exact Location of the Protein-Binding