What technique would you use to detect a target sequence in an intact chromosome using a labeled molecular probe?
A)PCR
B)in situ hybridization
C)western blotting
D)Sanger sequencing
E)karyotyping

Question

Quizplus · Accepted Answer

In situ hybridization is a technique that involves using a labeled molecular probe that is complementary to the target sequence. The probe is hybridized to the intact chromosome, allowing for detection of the target sequence. PCR amplifies specific sequences, but would not be suitable for detecting a target sequence within an intact chromosome. Western blotting detects proteins, not DNA sequences. Sanger sequencing is a method of sequencing DNA, but would not be applicable for detecting a specific target sequence within an intact chromosome. Karyotyping is a technique that involves visualizing chromosomes to detect any abnormalities, but would not allow for specific detection of a target sequence.

What Technique Would You Use to Detect a Target Sequence