Which of the following vectors would be most efficient to use to amplify fragments ~300 kb in length?
A)BACs
B)plasmids
C)YACs
D)lambda phage
E)cosmids

Question

Quizplus · Accepted Answer

YACs (yeast artificial chromosomes) are designed to accommodate large fragments of DNA (up to 1 Mb) and have been used successfully to clone fragments of up to 300 kb in length. BACs (bacterial artificial chromosomes) can also accommodate larger fragments, but YACs have a higher transformation efficiency in yeast and can be maintained as circular molecules, making them more stable than BACs. Plasmids, lambda phage, and cosmids are not suited for cloning fragments of this length.

Which of the Following Vectors Would Be Most Efficient to Use