Enhancer trapping might occur if you create a library of insertions bearing enhancer-less GAL4 genes. Their expression will be controlled by __________.
A)also inserting a viral enhancer
B)also inserting a GAL4 reporter
C)including a bacterial origin sequence
D)enhancers mapping near the insertion site
E)including an enhancer activator

Question

Quizplus · Accepted Answer

Enhancer trapping occurs when a gene without enhancers is randomly inserted into the genome, and its expression is controlled by the enhancers that are present nearby in the genome. Therefore, the best choice is D, which suggests including enhancers that map near the insertion site. Adding a viral enhancer or enhancer activator might increase the expression levels but would not necessarily trap enhancers. Including a GAL4 reporter or bacterial origin sequence is not relevant to enhancer trapping.

Enhancer Trapping Might Occur If You Create a Library of Insertions