P element transposition requires the enzyme transposase,which acts at the ends of the P element.To find transposons in a germ line,which of these is best?
A)use of a "crippled" P element that cannot transpose itself but can produce the enzyme
B)use of a "crippled" P element that can be transposed if there is another source of transposase
C)use of a P element that does not share the genome of the germ line
D)use of restriction sites that flank the inserted P element
E)use of DNA ligase to incorporate the P element

Question

Quizplus · Accepted Answer

Using a "crippled" P element that cannot transpose itself but can produce the enzyme transposase is the best option. This will allow for the transposase enzyme to be expressed without the risk of the P element itself moving and disrupting the genome. Therefore, this option allows for the targeted identification of transposons in the germ line.

P Element Transposition Requires the Enzyme Transposase,which Acts at the Ends