When a transgene is used in mice to knock out a specific function, two problems that might occur are the unintended introduction of multiple copies or abnormal gene expression. The second of these problems is related to __________.
A)position effect
B)homologous recombination
C)vector insufficiency
D)mitotic recombination
E)unequal crossing over

Question

Quizplus · Accepted Answer

Position effect refers to the way that the location of a gene within the chromosome can affect its expression. When a transgene is randomly inserted into the genome, its expression can be influenced by nearby regulatory sequences, leading to abnormal or unintended expression patterns. This is a common issue when using transgenic mice to knock out specific gene functions.

When a Transgene Is Used in Mice to Knock Out